1Division of Dairy Chemistry, ICAR-National Dairy Research Institute, Karnal-132 001, Haryana, India
2Dairy Chemistry and Bacteriology Section, ICAR-National Dairy Research Institute Southern Regional Station, Bengaluru-560 068, Karnataka, India
3Department of Food Technology, Faculty of Engineering and Technology, JAIN (Deemed- to- be University), Bangaluru-562 112, Karnataka, India
*Corresponding Author: Soma Maji, Department of Food Technology, Faculty of Engineering and Technology, JAIN (Deemedto be University), Bangaluru-562 112, Karnataka, India, Email: somadt08@gmail.com
Online published on 20 March, 2026.
The traditional Soxhlet method, known for its lengthy 8 to 16-hours extraction process according to AOAC (2005), prompted the exploration for a faster alternative to extract crude lipids from cow dung.
In this study, we compared and standardized three methods-Soxhlet, Mojonnier acid digestion and Folch method-for crude lipid determination. The dung sample of the Sahiwal cow breed was collected from the Live Stock Research Centre, NDRI-Karnal.
Different sample size, nature of sample, amount of HCL for digestion was used for standardization of the methods. The crude lipid content as determined by Folch method, Mojonnier acid digestion method and Soxhlet method was 3.37±0.50, 4.41±0.07 and 4.13±0.25 per cent, respectively. Results from Sahiwal breed dung samples showed significantly lower crude lipid content using the Folch method. Mojonnier method, particularly with dry samples, demonstrated comparable results to Soxhlet with an extraction time (1 to 1.3 hours), offering a viable alternative for rapid extraction of crude lipid.
Crude lipid, Dung, Extraction, Standardization, Validation
