Bulletin of Pure & Applied Sciences- Zoology
  • Year: 2020
  • Volume: 39a
  • Issue: 1

Assessment of microbial burden on vegetable salads for food safety and human health

  • Author:
  • Diwakar Aggarwal1, Sushil Kumar Upadhyay*,1, Lovelpreet Kaur1, Amit Kumar2, Harish Bhalla3, Raj Singh1
  • Total Page Count: 7
  • Published Online: Jun 1, 2020
  • Page Number: 130 to 136

1Department of Biotechnology, Maharishi Markandeshwar (Deemed to be University), Mullana-Ambala, Haryana, 133207, India

2Department of Biotechnology, College of Natural and Computational Sciences, Debre Markos University (Ethiopia)

3Biocell Institute of Biological Sciences, Patiala-147001, India

*Corresponding author: Dr. Sushil Kumar Upadhyay, Assistant Professor, Department of Biotechnology, Maharishi Markandeshwar (Deemed to be University), Mullana-Ambala, Haryana, 133207, India E-mail: sushil.upadhyay@mmumullana.org ORCID: https://orcid.org/0000-0002-1229-4275

Online published on 10 August, 2020.

Abstract

Plates of mixed greens salads have become fundamental piece of individuals’ eating routine all around the globe. They are normally consumed raw and with no treatment or careful washing; henceforth they have been accounted for to become vehicles for the transmission of pathogenic microorganism related with human diseases. Therefore, in present study fresh samples of salads consisting of various fruits and vegetable salads were collected from different areas in vicinity Maharishi Markandeshwar (Deemed to be University), Mullana-Ambala (HR), India and evaluated for bacterial loads using standard microbiological techniques. Most of samples showed presence of high amount mesophillic bacteria. Some samples further showed the presence of pathogens like Escherichia coli, Salmonella spp. and Staphylococcus spp., which were confirmed by using various biochemical test like triple sugar iron test, catalase test and sugar fermentation test etc. Most of microorganisms were found to be susceptible towards various antibiotics used in the study. The study may be helpful to policy makers in planning of food safety and health security in future for food borne disease free livelihood, sustainable development, economy and mankind.

Keywords

Escherichia coli, Salmonella spp, Staphylococcus spp, Antibiotic susceptibility test, Triple sugar iron test, Simmons citrate test, Catalase test, Food security, Human health