Bio Science Research Bulletin

  • Year: 2016
  • Volume: 32
  • Issue: 1and2

Photochemical Activation of Polyvinyl Chloride for Immobilization of Invertase

1Department of Zoology, Km. Mayawati Government Girls P.G. College, Badalpur, Gautam Buddha Nagar, Uttar Pradesh, 203207

2Department Innovative Diagnostics, CSIR-Institute of Genomics and Integrative Biology, Mall Road, Delhi-110 007, India

3New Idea Lab, C-202, Ramkrishnan Cooperative Group Housing Society, Plot-12, Sector 23, Dwarka, New Delhi, 110077, India

*Corresponding author. Email: azmi.naqvi@rediffmail.com

Online published on 21 January, 2017.

Abstract

Herein, we describe a method for immobilization of biomolecules onto polyvinyl chloride activated by 1-fluoro-2-nitro-4azidobenzene (FNAB) for a photochemical reaction. Immobilizing biomolecules onto the polyvinyl chloride surface were either very tedious to carry out or they require some specialized conditions and techniques for immobilization. Herein, we report a mild procedure for a covalent immobilization of enzymes using FNAB. HRP (Horse Radish Peroxidase) and invertase were chosen for demonstrating its efficacy onto activated polyvinyl chloride.

Keywords

1-fluoro-2-nitro-4-azidobenzene, Immobilization, Horse Radish Peroxidase, invertase, polyvinyl chloride