Are cytotoxicity tests in biological studies reliable?

Cytotoxicity is one of the most common tests used in biological studies to assay whether a compound has some, or any, kind of therapeutic properties on cell types. Although it is a widespread test across disciplines that range from pharmacy to biology and medicine, amongst others, there are factors that alter reliable outcomes. These factors are either laboratory or environmental; and has an effect on the intercellular and extracellular features cell types experience, once they are exposed to different substances. The fluctuations in cytotoxicity outcomes are a result of not only human error, but also the manner in which plants/compounds are prepared during preliminary experiments. However, it's possible for these tests to be conclusive by factoring the phytochemical constituents of plant compounds that have an effect on the genetics of signal transduction events in cells. This implies that the work performed on cytotoxicity is valid, however, several questions pertaining to the reliability of test outcomes still remain. In this paper, the trustworthy nature of cytotoxicity tests in biological studies will be considered.