1Department of Molecular Biology and Genetic Engineering, Bihar Agricultural University, Sabour, 813210
2Department of Plant Breeding & Genetics, Bihar Agricultural University, Sabour, 813210
3Department of Plant Pathology, Bihar Agricultural University, Sabour, 813210
*Corresponding Author, tribhuwanbau@gmail.com
Online Published on 07 September, 2023.
Extraction of quality RNA from xerophytic plants like Selaginella bryopteris for molecular biology applications is problematical due to the presence of many secondary metabolites. The existing protocols are not suitable to yield quality RNA in adequate quantity from fronds of Selaginella bryopteris. Therefore, most common method i.e. CTAB-based protocol was optimized and partially modified to solve the purpose. This modified protocol gave high quantity (500.00 μg/g fresh weight of leaf tissue) of quality RNA based on qualitative and quantitative analysis by spectrophotometer and electrophoresis respectively.
Selaginella bryopteris, RNA isolation, Xerophytic plant, Electrophoresis
