Plant Genome Construction of Molecular Maps, Functional Genomics and Comparative Genomics

Mapping genome of an organism is an important tool to provide a guide for the sequencing experiments by showing the exact positions of genes and other distinctive features in the chromosomal DNA. Whole genome sequencing of eukaryotic genomes is greatly facilitated by high- density genome maps. The high- density maps serve as foundation during organizing and assembling the nucleotide sequences of genomes. Distinctive features on the genome map are used as landmarks to aid assembly of master sequence from huge number of short sequences obtained from genomic library. First step for manipulation of genes for various applications is cloning that relies on mapping close to a convenient marker. A complete physical genetic map of genome is necessary for comparative genomics studies with other genomes. High resolution comparative physical maps will reveal regions of collinearity and rearrangement and will have important implications for phylogenetic studies and genome evolution.