Optimization of pectinase and cellulase production from microorganisms isolated from agriculture waste

Bacillus subtilis and Pseudomonas fluorescens were isolated as the pectinase and cellulase–producing bacteria from agricultural waste. Optimization of the fermentation medium for maximum pectinase and cellulase production was carried out. The culture conditions like pH, temperature, inoculum age and raw substrate were optimized. The optimum conditions found for pectinase and cellulase production were 37°C at pH 7 with agricultural substrate stimulates the production of pectinase and cellulase. Among bacteria, Pseudomonas fluorescens is the best cellulase and Bacillus subtilis is the best pectinase producer.