Biotech Today: An International Journal of Biological Sciences
  • Year: 2014
  • Volume: 4
  • Issue: 2

A Comparative Study Of Serum Proteins In Some Vertebrate Representatives

Department of Zoology and Biotechnology, H. N. B. Garhwal University, Srinagar, Garhwal, Uttarakhand

*Corresponding Author Email: singhyashpal13685@gmail.com

Online published on 19 September, 2015.

Abstract

Serum is the liquid part of blood, which can be analysed through protein electrophoresis (PEP) technique to determine the levels of different types of proteins in a blood sample. In present investigation a comparative electrophoretic study of serum protein of Gallus gallus domesticus, Hemidactylus frenatus, Schizothorax plagiostomus, Duttaphrynus melanostictus was carried out with serum fraction of man. There was no albumin like large fraction observed in Duttaphrynus metanostictus, Hemidactylus frenatus and Schizothorax plagiostomus. The number of serum protein fractions and their separation pattern differed in all these vertebrate representatives analysed. There were 20 fractions in man, 21 in Gallus gallus domesticus, 14 in Duttaphrynus melanostictus, 11 in Hemidactylus frenatus and 16 in Schizothorax plagiostomus.

Keywords

SDS- Sodium Dodecyl Sulphate, PEP- Protein Electrophoresis, PAGE- Polyacrylamide Gel Electrophoresis, Serum Fraction, Albumin, Globulins