1Department of Genetics and Plant Breeding, Banaras Hindu University, Varanasi, 221005, India
2Division of Genomic Resources, NBPGR, New Delhi-110012, India
3Department of Mycology and Plant Pathology, Banaras Hindu University, Varanasi, 221005, India
4CIMMYT South Asia, Kathmandu, Nepal
*Corresponding Author E-mail: sundeep@nbpgr.ernet.in
Online published on 18 June, 2016.
Genetic variation among 20 random iso-lates of Bipolaris sorokinian belonging to five mor-pho-pathological groups was analyzed by DNA finger printing based on RAPD analysis. Twenty random primers of different origin were used. Diag-nostic RAPD bands were generated from fungal DNA. UPGMA cluster analysis clustered the 20 isolates into two distinct groups. Of the 20 isolates, 19 were in the first group, while only one was in the other. An average genetic similarity between both the clusters was only 0.05 (5%). Isolate be-longing to different groups of morpho-pathological traits, were dispersed across several sub-clusters. In general, the classification based on morpho-pathological traits showed about 60% conformity with the grouping based on molecular markers. Re-sults illustrated the potential value of RAPDs for detecting polymorphism among isolates.
RAPD, Spot blotch (Bipolaris sorokin-iana), Genetic diversity, Isolates
