Induction Of Shoot Buds Multiplication Of Eusideroxylon Zwageri Tesym. and Binned (Borneo Ironwood) By Using Nodal Explants

Eusideroxylon zwageri is a tree of the tropical rainforest zone which is belongs to a family of Lauraceae. This species is one of the hardest timber tree species in Southeast Asia and endangered in some part of Southeast Asia. The objective of this study was to determine the optimal culture medium for the induction of shoot buds from nodal explants of E. zwageri. Different concentrations and combinations of BAP (1.0, 2.0, 3.0, 4.0, 5.0 and 6.0 mg/L) alone or either in combination with NAA (0.5 mg/L) or IBA (0.5 mg/L) were used to induce multiple shoot buds. The nodal explants collected from the healthy lateral branches of two to three years old of E. zwageri sapling. Finding showed that MS medium supplemented with 5.0 mg/L of BAP alone or in combination with either 0.5 mg/L of NAA or IBA had induced the highest mean number of shoots buds and leaves respectively. This is the first reported protocol on micropropagation of E. zwageri through induction of shoots multiplication by using nodal explants.