Department of Biochemistry, Panjab University, Sector-25, Chandigarh, India-160014
*Email Address: deeptichrungubch@gmail.com
Aspergillus niger, a black spored fungus produces enzyme trehalase, which is responsible for conversion of trehalose to glucose, it is responsible for spore germination. So by studying this enzyme's inhibition properties, one may learn to control germination of this fungus. This fungus is responsible for disease called black mould on certain fruits and vegetables such as grapes, apricots, onions, and peanuts, and is a common contaminant of food. So, in this study structure-inhibition analysis of trehalase was performed using tris-like compounds, and it was seen that Tris inhibits trehalase activity by 73%. And effect of different buffer systems was studied.
Aspergillus niger, Conidia, Optimization, Trehalase, Inhibition