Biotech Today: An International Journal of Biological Sciences
  • Year: 2017
  • Volume: 7
  • Issue: 1

Effect Of Different Nitrogen Sources on Poly-β-Hydroxybutyrate (PHB) Production

1U.P. Council of Agricultural Research, Lucknow

2Department of Genetics and Plant Breeding, Ch. Charan Singh University, Meerut

3Botany Departments, Guru Ghasidas University, Bilaspur, Madhya Pradesh

*E-mail: prabha11shashi@gmail.com

Abstract

The success in the biodegradable plastic approach mainly depends on the isolation, screening of bacteria, optimizing culture conditions for maximum PHB production and identification of PHB producing bacteria. The present study reports the isolation of bacteria, optimization of culture conditions for maximum PHB production and characterized for their morphological and biochemical properties. Out of a total of 54 isolated strains two strains were selected for PHB production in different culture conditions. When PHB production conditions were optimized with different nitrogen sources, the highest PHB production was observed in ammonium sulphate. The isolates were characterized biochemically as Bacillus species.

Keywords

Biodegradable plastic, PHB production and Bacillus species.