Biotech Today: An International Journal of Biological Sciences
  • Year: 2017
  • Volume: 7
  • Issue: 2

Effects of IAA and GA3 on In Vitro Root Regeneration in Potato Variety Kufri Chipsona-1 and Kufri Mohan

1Tissue Culture Laboratory, Deptt. of Agril. Biotechnology, S. V. Patel University of Agriculture & Technology, Meerut (Uttar Pradesh)

2Ministary of Agriculture, New Delhi

*Corresponding Author E-mail: alokkumar8483@gmail.com

Online published on 10 April, 2019.

Abstract

The present study was conducted to evaluate the effects of IAA and GA3 on in vitro root regeneration in two potato varieties Kufri Chipsona-1 and Kufri Mohan. Maximum (4.49) number of roots/shoot within a number of days (14.08), the longest (7.82 cm) root was observed with 1.0 mg/L IAA + 1.0 mg/L GA3 after 28 DAI in Kufri Chipsona-1. The main effects of GA3 in both varieties were observed with 1.0 mg/L GA3. The combined effects of IAA and GA3 were reported to be highest with 1.0 mg/L IAA + 1.0 mg/L GA3. Present study concluded that the presence of IAA in combination with GA3 in culture media was the most effective for high frequency root induction in both varieties of potato. Therefore, the present study recommends the use of IAA in combination with GA3 in culture media to achieve enhanced root induction and proliferation from shoots regenerated through in vitro induced callus in potato.

Keywords

Auxin, micropropagation, rooting, potato