Bioproduction of lactic acid by lactic acid bacteria exposed to Orotic acid monohydrate and 3-hydroxybutyric acid

The efficacy of orotic acid monohydrate and 3-hydroxybutyric acid on bioproduction of lactic acid by lactic acid bacteria such as Lactobacillus pentosus NCIM-2669, Lactobacillus casei NCIM-2732, Lactobacillus helveticus NCIM-2737 and Lactobacillus plantarum NCIM-2592 has been assessed. It has been observed that the bacterial strain Lactobacillus casei NCIM-2732 is most effective for the lactic acid fermentation process. It has been found that the compound, i.e., orotic acid monohydrate at molar concentration 5.0 × 10⁻⁵M enhances the bioproduction of lactic acid to an extent of 18.630% higher in comparison to control, i.e., 8.529g/100ml while at molar concentration 6.0×10⁻⁵M and onwards concentration of orotic acid monohydrate under trial inhibits and retards the bioproduction of lactic acid when experimental parameters are under optimized condition, viz. 34°C temperature, 6.0 pH, 6 days incubation period with 20% (w/v) molasses solution. On the other hand when the compound, i.e; 3-hydroxy butyric acid at molar concentration 6.00×10⁻⁵ M is incorporated in the same production medium it enhances the yield of lactic acid to an extent of 6.210% higher in comparison to control which is insignificant while at molar concentration 7.0×10⁻⁵ M and onwards of 3hydroxybutyric acid antagonises the lactic acid fermentation process thereby retards the yield of lactic acid.