Regulatory role of thiols and proline in mitigation of Cu induced phytotoxicity in seven day's old hydroponically acclimatized seedling of Cyamopsis tetragonoloba
Abstract
The present study investigates role of proline and thiols in alleviating the copper induced oxidative stress in Cyamopsis tetragonoloba var. RGC 936. Plants were subjected with different concentration of copper ranging from 10 μM to 100 μM for 96 hrs in Hoagland nutrient medium. Increasing concentration of copper drastically reduces plant growth, photosynthetic pigments, leaf water content, activities of antioxidants while increased malonaldehyde content and ROS. At sublethal concentration of copper (50 μM), remarkable improvement was observed in all these parameters. Accumulation of proline and thiols were 1.89 times and 1.45 times higher in leaves than control in Cu (50 μM) treated plants. The aim of current study to illustrate that potential role of proline and thiols against Cu induced phytotoxicity is mediated by regulating reactive oxygen species metabolism, cellular redox homeostasis and detoxification mechanism to heavy metal tolerance in seedlings of C. tetragonoloba.
Keywords
Copper stress, proline, thiols, reactive oxygen species, Cyamopsis tetragonoloba
