Correlation between the expression of bovine HSP70 and different biochemical parameters during thermal stress in cattle

Present study was aimed to characterize the stress response in native (Sahiwal) and crossbred (HF x Sahiwal: Frieswal) cattle using different biochemical assays. During the winter (15–18°C) and summer (42–45°C) months, blood samples were randomly collected from ten each native and crossbred cattle. Immediately after separation of plasma at different seasons, subjected for three biochemical assays viz. thio-barbituric acid reactive substances (TBARS), catalase (CAT) and glutathinoe peroxidase (GPX) assays. There was a significant (p<0.01) difference among breed and temperature with respect to CAT activity (nmol/min/ml). Plasma GPX level (nmol/min/ml) and MDA (μM) concentration was also significantly (p<0.01) higher during summer; however we could not achieved any significant variation among the breed. The fold changes of HSP70 gene was significantly (p<0.01) varies between the breeds and temperature. Association between the expression of HSP70 and the plasma biochemical profiles of enzymatic activities and MDA concentration revealed a highly positive significant (p < 0.01) correlation. Highest and significant (p<0.01) correlation was seen between GPX activity (69.8%) followed by MDA concentration (57.0%) and CAT activities (41.2%) with HSP70 expression. This study described the correlation between the expression level of HSP70 on the enzymatic activities during thermal stress in native and crossbred cattle.