Biotech Today: An International Journal of Biological Sciences

  • Year: 2018
  • Volume: 8
  • Issue: 1

Molecular characterization of Lentil (Lens culinaris Medikus) genotypes through Simple sequence repeat (SSR) markers

  • Author:
  • Ashish Kumar Pandey1,, Rakesh Singh Sengar1, Ashok Kumar2, Pooran Chand3, Raghbendra Yadav4, Vaishali1
  • Total Page Count: 8
  • Page Number: 65 to 72

1Department of Agricultural Biotechnology, College of Agriculture, Sardar Vallabhbhai Patel University of Agricultureand Technology, Meerut-250 110 (U.P.), India

2Department of Soil Science, College of Agriculture, Sardar Vallabhbhai Patel University of Agricultureand Technology, Meerut-250 110 (U.P.), India

3Department of Genetics and Plant breeding, College of Agriculture, Sardar Vallabhbhai Patel University of Agricultureand Technology, Meerut-250 110 (U.P.), India

4Department of Agronomy, College of Agriculture, Sardar Vallabhbhai Patel University of Agricultureand Technology, Meerut-250 110 (U.P.), India

Abstract

Lentil is a leguminous crop has an important role in human health and agriculture. Molecular markers are reliable tool to study genetic divergence in crop. The present study was conducted to assess genetic divergence in ten lentil genotypes through SSR markers. 19th SSR markers were selected under study in which nine markers were found to be polymorphic. A total of 26 discernible and reproducible bands including 25 polymorphic and 01 monomorphic bands were produced. The analysis revealed two distinct clusters within ten genotypes. PIC values ranged from 0.164 (SSR204) to 0.645 (SSR124) locus with an average of 0.424 for all loci. The range of genetic distance or coefficient of similarity among lentil genotypes varied 0.14 -0.58. The analysis of these similarities matrix revealed that greater similarity between DPL62 and PL04 (0.882), and lowest similarity between IPL406 and L4076 (0.188). In studied markers, three SSR makers (SSR19, SSR124, SSR130) exhibited maximum number of alleles and were found to be highly polymorphic. The outcome of this study will provide valuable information for breeding programs regarding genetic diversity.

Keywords

SSR, Genetic Diversity, Lentil, Polymorphism