Biotech Today: An International Journal of Biological Sciences

  • Year: 2018
  • Volume: 8
  • Issue: 2

Identification and characterization of Melanoidin Degrading Alkalohalophile Bacteria from Tainted Site of Molasses Distillery Effluents

  • Author:
  • Pallavi Mittal1,, Subhangini Sharma1, Madhu Kamle2, Ajay Bharti3, Vijay Tripathi4
  • Total Page Count: 6
  • Page Number: 51 to 56

1Department of Biotechnology, ITS Paramedical College, Ghaziabad, U.P-201 206, India

2Department of Forestry, North Eastern Regional Institute of Science & Technology, Nirjuli-791109, India

3Department of Civil Engineering, North Eastern Regional Institute of Science & Technology, Nirjuli-791109, India

4Department of Molecular and Cellular Engineering, Jacob Institute of Biotechnology and Bioengineering, Sam Higginbottom University of Agriculture Technology and Sciences, Prayagraj

*Corresponding Author e-mail: mittal_pallavi@yahoo.com

Online published on 22 August, 2019.

Abstract

The current research focused on isolation, identification of alkalohalophile the bacteria from the tainted soil samples of effluent treatment plant and their proficiency in degrading melanoidin. A total of four bacteria were selected from the total thirty five isolates and characterized on the basis of decolourization efficiency of melanoidin viz.; A5GS, A9GS, C5 and B9. Preliminary studies showed that A9GS showed 51.4% melanoidin degrading proficiency and identified as Enterobacter sp. as per biochemical studies. The isolate A9GS showed capability to grow on a wide range of salt (NaCl) concentrations ranging 0–10% and upto pH-11.0, indicating halotolerant and alkalophilic nature. Molecular characterization and phylogenetic analysis using 16S rDNA sequence analysis of A9GS showed the similarity to Enterobacter cloacae. To the best of our knowledge E. cloacae is the first report for the melanoidin decolorization from spent wash of molasses distillery effluents.

Keywords

Decolorization, Enterobacter, Biodegradation, soil management, phylogenetic