Application and Production of Biosurfactant from P. Aeruginosa

GS9–119, a strain of Pseudomonas aeruginosa was used in the present study for biosurfactant production. The active compounds were identified as rhamnolipids. A final medium for the production was designed in batch culture. In the presence of yeast extract, biosurfactant production was poor. For the nitrogen-source nitrate, which was superior to ammonium, an optimum carbon-to-nitrogen ratio of 18 existed. Further, the activity of biosurfactant is verified on the dirt and egg spots.