1Present address: Climate Change and Forest Influences Division, Forest Research Institute, P.O. New Forest, Dehra Dun-248 001, Uttarakhand, India
2Indian Grassland and Fodder Research Institute, Jhansi-284 003, (Uttar Pradesh), India
G.B. Pant University of Agriculture and Technology, Pantnagar-263145, (Uttarakhand), India
*Corresponding author Email: hukumsingh97@yahoo.com
Online published on 13 October, 2015.
Protein profiling of five rice genotypes, viz. KRH-2, Kasturi, Krishna Hamsa, Tulsi and Vasumati grown with four levels of nitrogen (0, 50, 100 and 200 kg ha−1) were evaluated in alluvial soil of Pantnagar, India during rainy season of 2008. Protein accumulation gradually increased in leaves and seeds simultaneously with increasing nitrogen doses. Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) was performed to separate protein and determine the molecular mass of samples in reference to the protein markers (97.4 kDa mass). The leaves profiling revealed the existence of two closely strongest protein bands with reference to protein marker of 54–57 kDa mass for all N rates in all rice genotypes. These bands resemble with Rubisco, the most abundant plant protein. No protein bands were found either in seed or in leaf to the reference of protein marker of 29 kDa mass for all cultivars at any N dose. The protein bands in seed samples were accumulated to the reference of protein marker for 66 kDa mass in all genotypes.
Nitrogen, Nitrogen use efficiency, Protein content, Protein marker, Rice genotypes, SDS-PAGE
