Effect of scarification on biochemical activities of aged seeds of guava (Psidium guajava)

The study was carried out on fresh, one, two and three years aged seeds of guava (Psidium guajava L.) L-49 (Sardar) in the laboratory of Department of Seed Science and Technology, CCS HAU, Hisar, during 2018–2019. The seeds were subjected to different seed scarification treatments, viz. hot water treatment at 80°C for 1 minute, concentrated sulphuric acid for quick dip and water soaking for 48 hr. The seeds were assessed for peroxidase activity, dehydrogenase activity and electrical conductivity. Hot water treatment at 80°C for 1 minute resulted into maximum peroxidase activity (710.0) and dehydrogenase activity (0.233) with minimum electrical conductivity (11.02). It is concluded that seeds treated with hot water treatment at 80°C for 1 minute showed maximum peroxidase, dehydrogenase activities and minimum electrical conductivity which reflects better seed quality.