* Department of Anatomy, Hassan Institute of Medical Sciences, Hassan, Karnataka, India.
** Department of Anatomy, S.R.M. Dental College, Deemed University, Ramapuram, Chennai, India.
*** Department of Anaesthesia, Vijayanagar Institute of Medical Sciences, Bellary, Karnataka, India.
**** Department of Physiology, Karnataka Institute of Medical Sciences, Hubli, Karnataka, India.
***** Department of Anatomy, Dr. ALM. PG. Institute of Basic Medical Sciences, University of Madras, Taramani Campus, Chennai, Tamilnadu, India.
****** Department of Anatomy, Basaveshwara Medical College and Hospital, Chitradurga, Karnataka, India.
*Correspondence to: Omprakash K.V. Department of Anatomy Hassan Institute of Medical Sciences Hassan 573201, Karnataka India.
Online published on 14 December, 2011.
The main aim of present study is to provide adequate numbers of cells to appropriate sites for useful cellular replacement to overcome the functional deficits caused by the trimethyltin chloride lesion. Hippocampal disorder was induced by the intraperitoneal administration of trimethyltin chloride (Sigma chemicals, U.S.A) at a single dose of 7.5 mg/kg body weight or two divided doses of 3.75 mg/kg body weight for two days. The Human Amniotic Epithelial cells to be transplanted were isolated from the fetal surface of the human placenta obtained from uncomplicated elective caesarian. Using standard coordinates human amniotic epithelial cells injected at four sites of hippocampal formation. For histological confirmations of the lesion and the growth of the transplant in the hippocampus, rat brains were processed and stained after various post operative periods of 7, 15, 30, 60,120 and 150 days. Trimethyltin chloride treatment caused a severe reduction in neuronal cell density in the CA1 and CA3 regions. The human amniotic epithelial cell grafts restored cell numbers in the above said regions to the near normal level. The present study concludes that the human amniotic epithelial cells may be used as a suitable donor tissue to alleviate various degenerative diseases in animal model before the clinical trial in humans, who are suffering from various degenerative diseases.
Dentate gyrus, Hippocampus, Human amniotic epithelial cells, Transplantation, Trimethyltin chloride
