In vitro regeneration of sesame (Sesamum indicum l.)-an important medicinal oil crop

Sesame (Sesamum indicum L.) cv. RT-54, the higher oil, protein content and very favourable additional food element is regenerated from hypocotyl explants through indirect organogenesis with callus phase. Hypocotyl segments of cultivar RT-54 of sesame were obtained frcm 10 days old in vitro raised seedlings through in vitro seed germination. In hypocotyl callus, maximum callus induction was achieved in Murashige and Skoog's medium supplemented with 3.0 mg/1 2, 4-dichloro phenoxy acetic acid. Higher frequency shoot regeneration (85%) was achieved in 6.0 mg/1 6-benzyl amino purine with 2.0 mg/1 napthalene acetic acid. Best shoot regeneration (85%) was achieved from 40 days old hypocotyl callus. Maximum number of shoots and shoot length can be achieved in 6.0 mg/1 BAP with 20% coconut water and 6.0 mg/1 6-benzyl amino purine with 0.5 mg/1 napthalene acetic acid. Highest concentration of 6-benzyl amino purine (8.0 mg/1) also showed maximum shoot length. During multiplication, callusing was also observed. Best rooting response (85–90%) and number of roots (8–10) were observed on 2.0 mg/1 indolyl-3-butyric acid. Whole plants recovered effectively and established successfully in soil to register 80–85% survival in the field.