Effect of 2,4-d on-callusing and morphogenesis of sugarcane (Saccharum spp. hybrid)

Tender leaf material of two prominent sugarcane cultivars viz., Co 99004 and CoN 05071 was cultured on MS medium augmented with various levels of 2,4-D (2,4-dichlorophenoxy acetic acid) for callusing and 6-BAP (6-benzyl amino purine) and NAA (Naphthalene acetic acid) for morphogenesis. Among different levels and combinations the 3 to 5 mg/1 2,4-D included in MS medium produced maximum dry, soft, compact and whitish callus within 18–30 days in both the cultivars. Whereas the 3 and 4 mg/1 BAP and NAa induced 81–100% morphogenesis or organogenesis within 27–35 days after culturing of both the varieties. The average number of shoots after organogenesis also varied with different levels of auxin like BAP and NAA. The combination 3 and 4 mg/1 BAP and NAA produced maximum number of shoots upto 10.8 to 12.5 in Co 99004 and 11.9 to 13.5 in CoN 05071. Whereas maximum length of shoot ranged upto 4.8 to 7.5 and 4.8 to 6.8, respectively.