1Department of Biochemistry and Microbiology, Biological Sciences Institute, São Paulo State University-Unesp, 13506-900 - Av 24A, 1515 Rio Claro, São Paulo, Brasil.
2Department of Food and Nutrition, Faculty of Pharmaceutical Sciences, São Paulo State University-Unesp, 13506-900 - Av 24A, 1515 Rio Claro, São Paulo, Brasil.
* For correspondence: jconti@rc.unesp.br
Some relatively low-cost supports were assessed for the immobilization of inulinase produced by the yeast strain Kluyveromyces marxianus var. bulgaricus. Crude enzyme in the culture supernatant was immobilized on various solid supports: activated carbon, diatomite, hen egg shell, Amberlite, porous silica and gelatin. The best performance was obtained in the gelatin-water support after treatment with glutaraldehyde as a crosslinking reagent, which showed an immobilization yield of 82.60% for inulinase specific activity.
Inulinase. Kluyveromyces marxianus, Fructose, Immobilization, Gelatin support
