Lab#302B, BRD School of Biosciences, Sardar Patel Maidan, Vadtal Road, Satellite Campus, Post Box No. 39, Sardar Patel University, Vallabh Vidyanagar-388120, (Gujarat), India.
* For correspondance: vasuthakkar@gmail.com
A Plackett-Burman design and statistical screening was employed to optimize medium components for jasmonic acid production by Lasiodiplodia theobromae. The seven medium components malt extract, sucrose, NaNO3, yeast extract, FeSO4 7H2O, MnSO4 H2O, and MgSO4 7H2O were screened using a Plackett-Burman design to optimize medium component for jasmonic acid production using L. theobromae. When sucrose was used alone as a carbon source with these medium components, the jasmonic acid production was found to be 80 mg/l. Increase in jasmonic acid production (225.3 mg/l) was observed with augmentation of malt extract and changing other medium components concentration based on statistical screening. It was found that malt extract, sucrose, NaNO3, MnSO4 7H2O, and MgSO4 7H2O were significant components influencing jasmonic acid production. Yeast extract and FeSO4 7H2O also showed significantly negative effect on the jasmonic acid production. Therefore significantly positive factors could be increased and significantly negative factors could be decreased for higher jasmonic acid production.
Jasmonic acid, Lasiodiplodia theobromae, Plackett-Burman design, Significant factors
