Micronutrients significantly influence both growth and production of the product by any microorganism. Taking this fact into consideration different micronutrients were screened for Vancomycin production using statistical design like Plackett-Burman. About 11 micronutrients including trace elements were independently screened in both chloride and sulphate forms using 12 experimental design of Plackett-Burman. The fermentative production of Vancomycin was studied for 10 days by bioassay method. The vancomycin yields were subjected to statistical analysis. Significant micronutrients in both chloride and sulphate form were short listed based on regression coefficients and tvalues. The micronutrients significant in sulphate form was lithium sulphate and in chloride form was magnesium chloride. These were short listed for further optimization studies as optimized production medium is a necessity for any commercial production. This screening strategy not only short listed the efficient diverse nutrients but also improved the antibiotic yields.
Amycolatopsis orientalis, Vancomycin, Plackett-Burman, Micronutrients
