Current Trends in Biotechnology and Pharmacy

Open Access
SCOPUS
  • Year: 2016
  • Volume: 10
  • Issue: 2

Production of Pullulan using Jaggery as substrate by Aureobasidium pullulans MTCC 2195

  • Author:
  • V S Rama Krishna Ganduri1,3, KRS Sambasiva Rao1, Usha Kiranmayi Mangamuri2, Vijaya M Lakahmi2, Sudhakar Poda1,
  • Total Page Count: 8
  • DOI:
  • Page Number: 153 to 160

1Department of Biotechnology, Acharya Nagarjuna University, Nagarjuna Nagar, Guntur-522510, Andhra Pradesh, India

3Department of Biotechnology, K. L. University, Green Fields, Vaddeswaram-522502, Guntur (Dist), Andhra Pradesh, India

2Department of Microbiology and Botany, Acharya Nagarjuna University, Nagarjuna Nagar, Guntur-522510, Andhra Pradesh, India

Abstract

Shake-flask fermentation, under batch cultivation, was investigated for the production of fungal exopolysaccharide, pullulan using jaggery (a traditional concentrated sugar cane juice) as a carbon substrate by Aureobasidium pullulans MTCC 2195. Change in the initial pH (from 3.0 to 7.0) of media containing jaggery was varied to study the effect of pH in the fermentation and maximum pullulan yield was obtained at a pH of 5.0. An increase in the initial concentrations (50, 75, 100 g/L) of jaggery in the media produced the maximum pullulan content as 21.6, 19.7 and 18.6 g per 100 g of jaggery, respectively, used. A sucrose based defined media were also used for comparison purposes. Fourier Transform InfraRed (FTIR) spectroscopic analysis was done to confirm the functional groups of synthesized pullulan and compared with that of commercial pullulan.

Keywords

Jaggery, Pullulan, pH, FTIR, Aureobasidium pullulans MTCC 2195