Current Trends in Biotechnology and Pharmacy
Open Access
SCOPUS
  • Year: 2016
  • Volume: 10
  • Issue: 4

In vitro Propagation for Rapid and Efficient Regeneration of Cardiospermum halicacabum L.-An important Medicinal Plant

  • Author:
  • Ashwani Kumar1,2,, S.C. Goyal1, Pooja 1, Jagdish Parshad3, Anshuman Singh2, Parvender Sheoran2, Charu Lata2, Rajkumar 2, Meenakshi Jain4
  • Total Page Count: 6
  • Page Number: 368 to 373

1Department of Botany & Plant Physiology, CCS HAU, Hisar-125004, Haryana, India

2ICAR-Central Soil Salinity Research Institute, Karnal-132001, Haryana, India

3Department of Microbiology, CCS HAU, Hisar-125004, Haryana, India

4Department of Molecular Biology and Biotechnology, CCS HAU, Hisar-125004, Haryana, India

*For correspondence: Ashwani. Kumar1@icar.gov.in

Online published on 27 July, 2017.

Abstract

Cardiospermum halicacabum, a medicinial plant rich in β-sitosterol, Dglucoside, saponin and quebrachitol. Direct regeneration was achieved only from the nodal explants which showed 100 per cent sprouting on MS medium supplemented with BAP (0.5 ppm) + NAA (1.0 ppm) in 12 days, while MS medium supplemented with BAP (0.5 ppm) + IBA (1, 2 or 3 ppm) showed early but decreased sprouting. The half strength MS basal medium without any growth regulator proved to be best medium for root induction for excised shoots. The in vitro raised plantlets were transplanted to mixture of sterile dune sand and FYM (3: 1) with moderate (80%) success.

Keywords

Cardiospermum halicacabum, nodal explants, axillary buds, growth regulators