Multiple Parameter Optimization for Maximization of Exopolysaccharide Production from Lactobacillus paraplantarum KM1 by Response Surface Methodology

Statistical experimental design was applied to optimize the medium for exopolysaccharides (EPSs) production from Lactobacillus paraplantarum KM1. A design comprising of incubation time, temperature, pH, carbon concentration (lactose) and nitrogen concentration (ammonium sulfate). A quadratic model was found to fit the EPS production. The optimum values of tested variables for EPS production were 25% w/v lactose, 35æ%C, pH 6.5 and 32 h for cultivation time. A great variation in EPS production was observed that ranged from 0 to 34.6 mg/ml. This culture condition enhanced EPS production. An overall increase of 40% was observed by optimizing the conditions through response surface methodology (RSM) in L. paraplantarum KM1. There was no significant difference between the predicted value and actual values of response were found and findings reflect the adequacy of RSM for optimization of EPS production by Lactobacillus paraplantarum KM1. The adequacy of this model for further analysis was possible since R² was greater than 0.70.