Bioparametric Investigation of Mutant Bacillus subtilis MTCC 2414 Extracellular Laccase Production under Solid State Fermentation

This work has been undertaken to investigate the bio parameters such as various substrates, initial moisture level, inoculum size, pH, incubation temperature, incubation period, metal ions and nitrogen sources effect on the production of laccase in solid-state fermentation using mutant Bacillus subtilis MTCC 2414. The laccase production was observed with a sesame oil cake (183.32 ± 0.29 U/g), initial moisture level 80% (189.28 ± 0.52 U/g), inoculum size 1.5% (196.12 ± 0.26 U/g), initial pH 8 (215.20 ± 0.48 U/g), incubation temperature 37°C (225.80 ± 0.52 U/g), incubation period 48h (258.80 ± 0.29 U/g), CuSO₄ (263.16 ± 0.12 U/g) and yeast extract (268.14 ± 0.16 U/g) in the production medium.