1Research Scholar of
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Nature is crucial source of drugs. Plants have played important role in identifying important drugs and are basis of various modern pharmaceuticals. Therefore ethnomedicinal plants must be exploited to identify lead compounds. C. acuminatum is an important plant of Astavarga (combination of 8 drugs, most commonly used in Ayurveda) C. acuminatum is used in breathing disorders, burning sensation, cough, decrease in bone tissue, bleeding disorders, blood disorders, tuberculosis, insect bites and rheumatism. It is refrigerant, febrifuge and aphrodisiac. It is utilized as tonic and in general debility. HPTLC is a valuable analytical tool for the investigation of herbal products and drugs. Hence, HPTLC Fingerprint profile has been developed for this plant.
The present study was aimed to develop the fingerprint profile by HPTLC of Crepidium acuminatum (D. Don) Szlach.
HPTLC System (CAMAG, Switzerland), equipped with linomat 5 applicator, development chamber, scanner, derivatizer, vision Cats software, was used. The plate was scanned at 580 nm using Tungsten lamp and images were captured at visible light, UV 254 nm and UV 366 nm.
HPTLC method for separation of phytoconstituents using different solvent system has been developed for C. acuminatum. The study revealed the presence of 2 saponins, 3 bitter principles, 2 steroids, 2 Sterols, 2 essential oils, 1 anthraquinones, 2 coumarin and 5 flavonoids in methanol extract of pseudobulbs of this plant.
The HPTLC fingerprint profile developed for methanol extract of C. acuminatum can be used for routine quality control of herbal formulations comprising of this plant and serve as a base for qualitative, quantitative analysis and standardization of the drug ‘Jeevak’. It will also help in identification and quantification of active/marker compounds. By isolating and identifying marker compounds, new drugs can be formulated to treat various diseases.
HPTLC, Fingerprint profile, C. acuminatum (Jeevak), Malaxis acuminata Astavarga, Phytochemical analysis
