Modelling and optimization of L-Asparaginase production from Bacillus stratosphericus.

L-Asparaginase has gained importance as one of the most important medications used to treat Acute Lymphocytic Leukemia (ALL). The goal of the current study is to maximize the L-Asparaginase activity besides minimizing the L-Glutaminase activity. Shorter half-life and side effects remain the drawbacks of the commercially available L-Asparaginase drugs. To overcome the drawbacks, L-Asparaginase has been isolated from endophytes of Ocimum tenuiflorum. The novel endophyte shown highest L-Asparaginase activity and it was identified as Bacillus stratosphericus using 16S rRNA sequencing analysis. With unoptimized medium, the maximum activity for L-Asparaginase was 7.81 IU/ml and for L-Glutaminase 6.51 IU/ml at 48h, pH-7.0 and temperature 25°C. It was observed that the L-Asparaginase activity was increased to 18 IU/ml by (One-Factor-at-a-Time) OFAT method. Further medium was optimized by Plackett-Burman method. It was observed that Time, Temperature, pH and L-Asparagine were identified as significant variables, which were further optimized by Response Surface Methodology (RSM). After RSM, the L-Asparaginase activity increased to 25.28 IU/ml whereas the L-Glutaminase activity diminished to 3.09 IU/ml.