Simple and rapid liquid chromatography-tandem mass Spectrometric (LC-MS/MS) method for the Simultaneous Determination of Pioglitazone and Glimepiride in human Plasma

A simple and rapid liquid chromatographytandem mass spectrometric (LC-MS/MS) was proposed for the simultaneous determination of pioglitazone and glimepiride in human plasma. The analytes and the internal standards (IS) were extracted from 100 μL aliquots of human plasma via solid phase extraction (SPE). A mobile phase composed of 5 mM ammonium acetate (pH 3.0) and methanol (20: 80, v/v) was used to chromatograph the analytes on a C₁₈ column. The calibration curve obtained was linear (r² 0.999) over the concentration range of 12.15–2418.27 ng/mL for pioglitazone and 2.00–500.61 ng/mL for glimepiride. The total run time was rapid with 2.5 min. and can analyze more samples in a day. All the validation results were complying with the recent USFDA guidelines.