1Department of Biotechnology, Vignan's Foundation for Science, Technology & Research, Valdamudi-522213, India
2 Department of Biotechnology, Vikrama simhapuri University, SPSR, Nellore-524003, India
3Department of Biotechnology, Acharya Nagarjuna University, Guntur-522510, India
4Departemnt of Botany, Rayalaseema University, Kurnool-518002, India
*Correspondence: kodalividyaprabhakar@gmail.com
Online published on 30 December, 2019.
Plastics and polypropylene polymers are synthesized from nonrenewable resources and persist in environment long after intended use, resulting into problems of global environmental pollution. Hence, the present study focused on production of polyhydroxybutyrate (PHB) microbial polyester by bacterial fermentation. In the present study, 05 PHB producing bacterial species were isolated from sewage waste, Guntur, India. Among all one isolate showed the maximum PHB yield of 4g/L. The high PHB producing bacterium was identified as Acinetobacter nosocomialis RR20, based on biochemical and molecular methods. Further, the characterization of PHB produced from this strain was also studied by analytical methods namely, FT-IR, DTA, TGA, 1H NMR, 13C NMR and LC-MS.
Poly-3-hydroxybutyrate (PHB), Acinetobacter nosocomialis RR20, FT-IR, TGA, DTA, NMR, LC-MS
