Molecular Characterization of a Biopolymer Producing Bacterium Isolated from Sewage Sample

Plastics and polypropylene polymers are synthesized from nonrenewable resources and persist in environment long after intended use, resulting into problems of global environmental pollution. Hence, the present study focused on production of polyhydroxybutyrate (PHB) microbial polyester by bacterial fermentation. In the present study, 05 PHB producing bacterial species were isolated from sewage waste, Guntur, India. Among all one isolate showed the maximum PHB yield of 4g/L. The high PHB producing bacterium was identified as Acinetobacter nosocomialis RR20, based on biochemical and molecular methods. Further, the characterization of PHB produced from this strain was also studied by analytical methods namely, FT-IR, DTA, TGA, ¹H NMR, ¹³C NMR and LC-MS.