Biochemical characterization and dye decolorization of selective microbial isolates from natural sources

The estimation of dye decolorizing efficiency of microorganisms was initially carried out by the primary screening of isolates from the soil samples of Ambabari region Jaipur. Two microbial Strains Bacillus subtilis and Psedomonas stutzeri were isolated from the soil samples. Dye decolorizing efficiency was checked by plating and shake culture flask experiments, provided with the optimized conditions of temperature/time period. Selective isolated microbial strains were further analyzed spectrophotometrically for decolorization percentage, morphological analysis carried out by visualizing the colony parameters with respect to their size/shape or color. Selective microbial isolate Bacillus subtilis was found with the capability of 73.52% to decolorizie1 ppm methyl red in 96 h and 79.71% capability to decolorize 100 ppm methyl red in 96 h. The same isolate Bacillus subtilis was found with the capability of 74.64% to decolorize the 1 ppm orange G in 96 h and 80.80% capability to decolorize the 100 ppm orange G in 96 hrs. Pseudomonas stutzeri was found with the capability of 70.16% to decolorize 1 ppm methyl red in 96 h and 77.17% capability to decolorize 100 ppm methyl red in 96 h. Microbial isolate pseudomonas stutzeri was having capability of 72.80% to decolorize the 1 ppm orange G in 96 h and 75.81% capability to decolorize 100 ppm orange G.