Optimization of process parameters for high yield production of exo-inulinase from Trichoderma asperellum RSBR08 by using solid state fermentation

Inulinase is an industrially applicable enzyme which is used widely in the production of ultra high fructose syrups and fructooligosaccharides. Production of inulinase enzyme in a cost effective manner is the major challenge faced. In the present research study, Trichoderma asperellum RSBR08 which has the capability to produce high exo-inulinase enzyme is produced by using the solid state fermentation. Process parameters for production of exo-inulinase by using solid state fermentation were optimized. Results showed high exo-inulinase production in garlic as substrate (199.2±6.3 U/gds), 45% moisture percentage (228.4±3.4), 26°C temperature (219.1±5.8) and 5.0 pH (213.6±4.5). Effect of different metals viz. Mg²⁺, Zn²⁺, K⁺, Ca²⁺, Na⁺, Mn²⁺ and Hg²⁺ were studied on the production of exo-inulinase enzyme in which Ca²⁺ induced the enzyme production (196.3±5.6) whereas Hg²⁺ has inhibited the inulinase production (37.0±3.3). Based on the above results, the solid substrate fermentation parameters optimized can be used industrially.