1Department of Chemistry, V.S.R Government Degree & P.G. College, Movva, Andhra Pradesh, India
2Department of Chemistry, Acharya Nagarjuna University, Nagarjuna Nagar, Guntur, Andhra Pradesh, India
*Corresponding author: chemistryprasad.n@gmail.com
Online published on 27 May, 2021.
A new analytical “reverse phase high performance liquid Chromatography (RP-HPLC) assay method has been developed for estimation of” Levofloxacin in injection phase. The separation was achieved by using column Inertsil ODS-3V (250 x 4.6mm, 5μm) mobile phase consisted of 0.05 M solution of citric acid monohydrate and 10 ml of 1.0 M ammonium acetate buffer and acetonitrile in the ratio of (85: 15 v/v). The flow rate was 1.0mL.min-1. Levofloxacin was detected using UV detector at the wavelength of 293 nm. The retention time of Levofloxacin was noted to be 11.20 min respectively. The method was evaluated as per ICH guidelines. The proposed method was found to be advantageous than the existing methods towards accuracy, reproducibility, and consistent.
RP-HPLC, Levofloxacin, Forced degradation and Validation
