1Department of Life Sciences, CHRIST (Deemed to be University), Bengaluru-560029
2DR Biosciences, Research and Development Unit, Bettahalasur, Bangalore-562157
3Department of Biotechnology, REVA University, Yelahanka, Bangalore-560064
*Corresponding author: venkatanagarajue@gmail.com
Online published on 4 July, 2024.
Microorganisms that degrade oil contribute significantly to the bioremediation of polluted lakes. Many microorganisms synthesize lipases, which are commercially significant. In the present study microorganisms producing extracellular lipase were isolated from various polluted lakes in Bangalore by using tributyrin agar. A lipase assay was done to determine the most efficient lipase-producing organism, which was then named Bacillus mojavensis CUIE1819 based on 16srRNA sequencing. After UV irradiation, the selected immobilized organisms were used to treat the lake water samples.
Microbial Lipase, Screening, Production, Lipase assay, Oil degradability, Mutagenesis