Current Trends in Biotechnology and Pharmacy
Open Access
SCOPUS
  • Year: 2022
  • Volume: 16
  • Issue: 4

Non-recombinant mutagenesis of Bacillus mojavensis CUIE1819 for hyper production of lipase and treatment of polluted lakes

  • Author:
  • J Irene Infancy1, Erumalla Venkata Nagaraju2,*, Vaddi Damodara Reddy3, Kuppusamy Alagesan Paari3
  • Total Page Count: 10
  • Page Number: 471 to 480

1Department of Life Sciences, CHRIST (Deemed to be University), Bengaluru-560029

2DR Biosciences, Research and Development Unit, Bettahalasur, Bangalore-562157

3Department of Biotechnology, REVA University, Yelahanka, Bangalore-560064

*Corresponding author: venkatanagarajue@gmail.com

Online published on 4 July, 2024.

Abstract

Microorganisms that degrade oil contribute significantly to the bioremediation of polluted lakes. Many microorganisms synthesize lipases, which are commercially significant. In the present study microorganisms producing extracellular lipase were isolated from various polluted lakes in Bangalore by using tributyrin agar. A lipase assay was done to determine the most efficient lipase-producing organism, which was then named Bacillus mojavensis CUIE1819 based on 16srRNA sequencing. After UV irradiation, the selected immobilized organisms were used to treat the lake water samples.

Keywords

Microbial Lipase, Screening, Production, Lipase assay, Oil degradability, Mutagenesis