Environmental Bioremediation of DDT-Contaminated Soil by Using the Mushroom Extract of L. edodes

Fungal extract of L. edodes was evaluated to degrade DDT (dichlorodiphenyltrichloroethane). Two grams of the dry weight of soil was artificially contaminated with 50 μL of 5 mM DDT in Dimethyl formamide (final amount: 0.25 μmol). The DMF evaporate was allowed to room temperature for 3 days and then mixed two grams of dry-weight fungal extract of L. edodes was added to the contaminated soil. The samples were tested and analyzed by gas chromatography. Manganese peroxidase activity, lignin peroxidase activity, and laccase activity of fungal extracts were analyzed. The fungal extract significantly degraded DDT in the mixture. It was also observed that the extract has higher ligninolytic enzyme activity which has played a role in DDT degradation.