Protective Effect of Annona Squamosa Fruit Pulp on Motor Responses Following Intra-Medial Forebrain Bundle Injection of 6-Ohda In Rat Model of Parkinson Disease

The present study investigated the ethanolic extract of fruit pulp of Annona squamosa that ameliorates the 6-OHDAinduced behavioural, biochemical, and neurochemical changes which resemble Parkinson’s disease (PD)-like symptoms.

Various behavioural and biochemical parameters were carried out to evaluate the activity of ethanolic extract of fruit pulp of Annona squamosa (FEAS) on 6-OHDA treated rats. To determine the therapeutic significance of FEAS on PD, different behavioural tests such as apomorphine rotation, narrow beam maze, rotarod, grip strength, sensorimotor and disengage test and some biochemical tests along with neurochemical findings were done.

6-OHDA caused physical and behavioral abnormalities in animals, including abnormal posture, weak grip strength, and motor deficit. Biochemical analysis of brain homogenates in FEAS treated rats showed altered oxidative stress and elevated lipid biomarkers. Neurochemical alterations of the striatum of FEAS treated rats exhibit altered levels of catecholamines. FEAS administered for 07 days significantly enhanced motor function and behaviour tasks and further restored the invitro antioxidant changes in the brain. Furthermore, FEAS-II treatment significantly improved oxidative damage, which is denoted by the alterations in neurochemical changes of rat brain.

In this research work, FEAS-I & II (200 mg/ kg & 400 mg/kg) provided a remarkable neuroprotective impact, which was evidenced by behavioural and biochemical tests. It restored the behavioural and biochemical alterations caused by 6-OHDA and confirmed the strong neuroprotective mechanism of FEAS in 6-OHDA-intoxicated behavior and motor abnormalities.