Antihyperglycemic Effect of Annona squamosa Leaf and Oleanolic Acid Combination in Diabetic Albino Rats

Since form ancient time, plant derived product has been used as holistic approach in the control of diabetes mellitus. The object of the study is evaluation of the synergistic anti-hyperglycemic effect of medicinal plant Annona squamosa leaf extract (ASLE) and plant-derived oleanolic acid (OA) in streptozotocin (STZ)-induced diabetic rats. Thirty six number of Wistar rats are taken and divided within six groups (n = 6). The group 1 is normal control, group 2 is diabetic control induced with STZ, groups 3, 4, 5, and 6 treated with 50 mg/kg of ASLE, 50 mg/ kg of OA, the combination of both 25 mg/kg of ASLE and 25 mg/kg of OA, and 0.1 mg/kg of glimepiride respectively for 28 days. The parameters like body weight, blood glucose, insulin, glycosylated hemoglobin, C-peptide, serum biochemical, lipid profiles and liver antioxidant parameters were evaluated. Results shows significant (p < 0.05) restoration of body weight, insulin and C-peptide levels. The animal groups treated with the combination of both ALSE and OA have experienced a remarkable restoration of serum biochemical, lipid profiles, antioxidant parameters, and C-peptide level as compared to the above groups due to the synergistic effect. These findings conclude that combination of both ASLE and OA had a synergistic action and it shows more potent anti-hyperglycemic effect.