ISAS – Institute for Analytical Sciences at the Technical University of Dortmund, Bunsen-Kirchhoff Strasse 11, D-44139, Dortmund, Germany Phone: +49 231 1392 215; Fax: +49 231 1392 120
*For Correspondence-heise@isas.de
The processes involved in bioreactors are complex and a detailed monitoring of the chemical composition of gaseous, liquid and solid matter involved in the bioprocess is of crucial importance for efficient operation. However, only a few parameters can be reliably monitored in real time with commercially available instrumentation, but there is a growing demand and need for effective and efficient process monitoring tools. Infrared (IR) spectroscopy has proved to be a powerful method for the study of various biomedical and bioanalytical applications. In this paper, applications of this analytical method for bioprocess monitoring have been discussed by reviewing the relevant literature from the last ten years. Furthermore, recent advances in our laboratory in the field of bio-reactor monitoring by use of mid-IR attenuated total reflection (ATR) spectroscopy and the development of mid-IR sensor systems for continuous biofluid monitoring based on transmission spectroscopy have also been addressed.
Infrared spectroscopy, Bioprocess monitoring, Near-infrared spectroscopy, Midinfrared spectroscopy
