1J.J. College of Arts and Science, Pudukkottai-622 422, Tamilnadu, India.
Thiagarajar College (Autonomous), Madurai-625 009, Tamilnadu, India.
*For correspondence - jothibasu77@gmail.com
†Present Address: Alagappa University, Karaikudi-630 003, Tamilnadu, India
A protocol was developed for in vitro propagation by multiple shoot induction of Crataeva religiosa Hook. f. & Thoms, a medicinal tree having high medicinal values belonging to the family Capparidaceae. High frequencies of multiple shoot regeneration were achieved from apical bud on MS medium fortified with 8 mg/L BAP alone. Five to seven shoots per explant were obtained. The elongated shoots were subcultured for rooting on half strength MS supplemented with various concentrations of IBA and IAA. The in vitro raised plantlets were acclimatized in green house and successfully transplanted to natural condition with 72% survival.
Crataeva religiosa, Medicinal plant, Micropropagation, Acclimatization
