1Bioengineering and environmental center, Indian Institute of Chemical Technology, Hyderabad, Andhra Pradesh, 500 007India
2Organic chemistry-1, Indian Institute of Chemical Technology, Hyderabad, Andhra Pradesh, 500 007India
For correspondence: prakasam@iict.res.in
The Present study reveals the effect of Lactobacillus delbrucekii cells immobilized in various matrices for the production of optically pure L(+) lactic acid. Functionalized alginate matrices were effective and suitable for higher L (+) lactic acid yields compared to other matrices. Repeated batch fermentation showed productivity of 1.74, 1.44, 1.48 and 1.52 Y p/s with functionalized alginate, Ca-alginate, K-Carrageenan, and glass beads, respectively. L. delbrucekii cells were immobilized in natural and functionalized alginate beads. The scanning electron microscopic studies showed increase in entrapped microbial cell biomass in modified immobilized beads compared to other matrices. These modified alginate beads showed enhanced stability and selectivity towards L (+) lactic acid production in higher yields with an enantiomeric selectivity of 99% and low by-product production.
Alginate, Enantiomeric selectivity, Fermentation, Immobilization, L(+) lactic acid, Lactobacillus delbrucekii
