1Department of Biochemistry, Yuvaraja's College, University of Mysore, Mysore-570005, Karnataka, India
2Plant Cell Biotechnology Department, Central Food Technological Research Institute, Mysore-570 020, Karnataka, India
3Department of Studies in Biochemistry, Kuvempu University, Tolahunase-577 002, Davangere, Karnataka, India
4Department of Studies in Biochemistry, University of Mysore, Mysore-570 006, Karnataka, India
Secoisolariciresinol diglucoside (SDG) is an important lignan found in flaxseed and is an emerging source in the functional food area. In the present study, antibacterial properties of SDG extracts from hull, endosperm and flour fractions of Indian flaxseed (Linum usitatissimum) varieties (LVF-01 and GVF-03) were evaluated. The SDG extracts were tested against the six bacterial species Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus subtilis, Agrobacterium tumefaciens, Bacillus cereus, and Escherichia coli. The maximum SDG and phenolic contents were found to be 16.9 and 12.5 mg/g and 3.18 and 2.70 mg/g in hull fractions of LVF-01 and GVF-03 respectively, when compared to flour and endosperm fractions. Among the fractions, the hull fraction of LVF-01 showed maximum activity 31.5 mm at MIC 100 ppm against E. coli, while minimum inhibitory activity was 3.1 mm with MIC at 300 ppm against B. subtilis. Similarly, in the case of GVF-03, maximum activity (31.9 with MIC 150 ppm) of the hull fraction, whereas, its minimuminhibitory
Flaxseed, lignans, SDG extracts, phenolics, HPLC, antibacterial properties
