Current Trends in Biotechnology and Pharmacy
Open Access
SCOPUS
  • Year: 2010
  • Volume: 4
  • Issue: 3

Expression of a bacterial chitinase (ChiB) gene enhances antifungal potential in transgenic Litchi chinensis Sonn. (cv. Bedana)

  • Author:
  • D.K. Das, A. Rahman
  • Total Page Count: 14
  • Page Number: 820 to 833

Post Graduate Department of Biotechnology, T.M.Bhagalpur University, Bhagalpur-812007, Bihar, India

*For Correspondence -dilipdas1@live.com

Abstract

Transgenic litchi harboring chitinase and gus genes were generated via Agrobacterium mediated transformation of zygotic embryos. Confirmation of the transgenic was done by PCR, RT-PCR, Southern blotting and by Western blot. Functional analysis of the transgenic was also attempted

Keywords

Agrobacterium tumefaciens, Somatic embryogenesis, die back, leaf spots and blight, Transformation, Phomopsis sp.