Current Trends in Biotechnology and Pharmacy

Open Access
SCOPUS
  • Year: 2010
  • Volume: 4
  • Issue: 4

RP-HPLC Method for the Estimation of Montelukast Sodium in Pharmaceutical Dosage Forms

  • Author:
  • J.V. Shanmukha Kumar1,, D. Ramachandran2, S. Vijaya Saradhi3
  • Total Page Count: 4
  • DOI:
  • Page Number: 943 to 946

1Dept. of Chemistry, College of Engineering, K.L.University, Vaddeswaram – 522 502, Guntur, A.P, India.

1Dept. of Biotechnology, College of Engineering, K.L.University, Vaddeswaram – 522 502, Guntur, A.P, India.

3Dept. of Chemistry, Acharya Nagarjuna University, Nuzvid Campus, Nuzvid, Krishna District, A.P, India.

*For Correspondence - shanmuk_fed@klce.ac.in

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Abstract

A reverse phase high pressure liquid chromatographic method (HPLC) has been described for the estimation of Montelukast Sodium in its pharmaceutical formulations using an inertsil ODS C-18, 5 μm column having 250×4.6 mm I.D. in gradient mode, with mobile phase A, containing 0.02 M sodium phosphate buffer: methanol (85:15) and mobile phase B, containing acetonitrile: methanol (85:15), at different time intervals. The flow rate was 1.0 ml/min and effluent was monitored at 218 nm and the linearity was found to be in the range of 0.1 to 10 ìg/ml. The method is simple, precise, specific, less time consuming and accurate for the estimation of montelukast Sodium in Pharmaceutical dosage forms.

Keywords

HPLC, Montelukast Sodium, Pharmaceutical Dosage Forms, Retention time, Linearity