Optimal Production Conditions, Cultural and Physiological Characterization of an Active-inhibitory Compound(s) Producing Streptomyces Bb36Isolate against Multiresistant Bacterial pathogens

A Streptomyces Bb₃₆ isolate was found to inhibit multi-drug resistant bacterial pathogens. When the activity of Bb₃₆Streptomyces isolate was compared to 23 standard antibiotics, data revealed that this isolate may not produce any of these antibiotics except cefotaxime and cefuroxime sodium. Bb₃₆ isolate began to produce inhibitory bioactive compounds against Escherichia coli and Staphylococcus aureus after 5 days and optimally in yeast-extract maltextract (YEME) broth at 28°C with inhibition zone diameter = 8 mm. Ethanol extraction and evaporation of the culture filtrate revealed activity against E. coli and S. aureus at MIC of 10 and 5 mg ml⁻¹ of the crude residues of evaporated ethanol extract with 8 and 11 mm inhibition zone diameter, respectively.