Enhanced Hyaluronic Acid Production by a Mutant Strain, 3523-7 of Streptococcus zooepidemicus

Hyaluronic acid (HA) is a hydrated gel and comprises repeating units of glucuronic acid and N-acetylglucosamine. HA has been of considerable interest due to its clinical application. This study reports the optimization of fermentation conditions for HA production by Streptococcus zooepidemicus 3523-7, a mutant derived from MTCC 3523. In shake flask fermentation, CDM containing 2% glucose and 1.5% yeast extract at pH 7.2 and temperature 36°C with 1% inoculums favored maximum HA production by the strain 3523-7. Enhanced HA production (1.89 g/l) by 3534-7 was seen when the fermentation was carried out in a 10 L bioreactor under optimal conditions such as 400 rpm and aeration 0.6 vvm.